The Fastest Western in Town: A Contemporary Twist on the Classic Western Blot Analysis

The fastest Western in town: a contemporary twist on the classic Western blot analysis.

The Western blot techniques that were originally established in the late 1970s are still actively utilized today. However, this traditional method of Western blotting has several drawbacks that include low quality resolution, spurious bands, decreased sensitivity, and poor protein integrity. Recent advances have drastically improved numerous aspects of the standard Western blot protocol to prod...

Understanding the Western Blot

Inquiries about various issues relating to Western blot (WB) testing are frequently posted to the Lyme disease discussion groups on the Internet. Among the most commonly asked questions are: What laboratory techniques are used to carry out the assay? What exactly is being measured? What is a “band”? How are the results interpreted? What are the CDC criteria for a “positive” test? Although some ...

Relative expression of fibroblast growth factor-1 in the cerebrospinal fluid of patients with bacterial meningitis; A Western Blot analysis

Meningitis is an inflammation of the membranes that surround the brain and spinal cord, thereby involving the arachnoid, the pia and the cerebrospinal fluid (CSF). It is divided into viral and bacterial meningitis. For different reasons the diagnosis of bacterial meningitis is very important. The examination of CSF samples may provide information about causative microorganism. The sensivity of ...

Western blot analysis in patients with hypocaeruloplasminaemia.

Inherited copper toxic disease, Wilson's disease, is an autosomal recessive disorder arising from a defect in biliary copper excretion. Although there are several pathognomonic clinical features, such a multisystem disease can be difficult to diagnose, particularly in the early stages of copper toxicity. Even measurements of serum copper and caeruloplasmin, the major copper-transporting protein...

The fallibility of HIV western blot.